Influence of N6o/PVP complex on the healing of wounds and the toxisity in the experiments in vivo

V.A. Popov^, M.A. Tyunin^, O.B. Zaitseva^, N.V. Sirotinkin?, and L.B. Piotrovsky*
^Military Medical Academy, St.-Petersburg, Russia Technical University (Technological Institute), St.-Petersburg, Russia
*Institute of Experimental Medicine RAMS, St.-Petersburg, Russia

Main part of the published data concerning the biological activity of fullerence deal with the results of investigations in vitro, and only several works describe the experiments in vivo, for the whole organism.

Taking into account for the wound process the increase of the content of the products of peroxide oxidation of lipids and free radicals, which possess the injuring effect, it seemed reasonable to study the influence of fullerene C60 on the healing of wounds. The obtained data concerning the proportionality of antioxidant activity of N6(/PVP complex to its concentration allowed us to chose precisely this water-soluble form of fullerene N60 for the further investigations.

White rats of the Wistar line (the weight 160-180 g were used in the experiments. Two methods of application were used: either the powdering of the dry N6o/PVP complex on the wound, or its injection into the spongy wound covering. The influence of N60/PVP complex on the wound healing has been studied at aseptic tangential, full-thickness skin, purulent wounds, as well as for granulation wounds after deep thermal burns. It was found that N6(/PVP complex stimulates actively rehabilitation processes in the wound (the formation of granulations, marginatum epithalamica, cicatrisation) and decreases the wound healing for 30%. These data allows us to make the conclusion about the perspectives of using N60/PVP complex as a wound healing preparation.

That is why the activity of this complex was also estimated for intra-abdominal injection of its 10% and 25% aqueous solution to rats. Structure-morphological changes and the activity of enzymes were investigated in dynamics in viscera of abdominal and thoracic cavities, kidneys and brain. It was found that N60/PVP complex does not show general toxicity - does not change the activity, the weight of the animals and their general state.

The obtained data testify the validity and perspectives of the use of N6o/PVP complex, immobilized in wound coating, in purulent surgery and the surgery of deep burns.