High-performance liquid chromatography

    HPLC is one of efficient methods of analyzing and separating complex mixtures.  The principle of chromatographic separation also forms the basis of a number of technologies.

    As an analysis method, the HPLC is included in the group of methods, which, due to the complexity of the investigated objects, involve preliminary separation of a primary complex mixture into relatively simple ones.  The obtained simple mixtures are then analyzed by simple physicochemical methods of by special methods developed for chromatography.

     The principle of liquid chromatography consists in the separation of the mixture components based on the difference in their equilibrium distribution between two immiscible phases: immobile phase and mobile one.

    The distinctive feature of the HPLC is using the high pressure (up to 400 bar) and slurries (usually 3-5 mcm, now as small as 1.8 mcm).  This makes it possible to separate complex mixtures of substances quickly and completely (average analysis time is 3-30 minutes).

There are other methods of analysis of nanomaterials:

• Mass spectrometry
• Ir-spectrometry
• X-ray structural analysis
• Spectrofotomery in ultra-violet and visible areas of the spectrum
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